Simultaneous determination of catecholamines and unconjugated 3,4-dihydroxyphenylacetic acid in brain tissue by ion-pairing reverse-phase high-performance liquid chromatography with electrochemical detection |
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| Authors: | Ivan N. Mefford Mark Gilberg Jack D. Barchas |
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| Affiliation: | Nancy Pritzker Laboratory of Behavioral Neurochemistry, Department of Psychiatry and Behavioral Sciences, Stanford University School of Medicine, Stanford, California 94305 USA |
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| Abstract: | A rapid, sensitive method was developed for the simultaneous assay of catecholamines and 3,4-dihydroxyphenylacetic acid in rat brain tissue. The method is simple, involving only tissue disruption, adsorption of the catechols onto alumina, desorption, and injection into a reverse-phase high-performance liquid chromatography system. Selectivity and high sensitivity are obtained using electrochemical detection. The addition of 3,4-dihydroxyphenylacetic acid determination to assays for catecholamines allows one to observe effects of pharmacological maniqulations on in vivo monoamine oxidase activity and/or turnover of dopamine as well as effects on catecholamine concentrations. |
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| Keywords: | hplc high-performance liquid chromatography LCEC hplc coupled with electrochemical detection HVA homovanillic acid DOPAC 3,4-dihydroxyphenylacetic acid VMA vanillylmandelic acid CSF cerebrospinal fluid NE norepinephrine E epinephrine DA dopamine DHBA dihydroxybenzylamine MAO monoamine oxidase |
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