The interaction of amino acids with o-phthaldialdehyde: A kinetic study and spectrophotometric assay of the reaction product |
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| Authors: | Vytas-JK ?vedas Igor J Galaev Ivan L Borisov Ilya V Berezin |
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| Institution: | 1. Chemical Department Lomonosov State University, Lenin Hills, Moscow 117234, USSR;2. A. N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Lomonosov State University, Lenin Hills, Moscow 117234, USSR |
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| Abstract: | A detailed study has been made of the kinetics of interaction between amino acids and esters of amino acids and o-phthaldialdehyde in the presence of mercaptoethanol. The reaction products have been characterized. A spectrophotometric method for quantitative analysis of all amino acids, except proline and hydroxyproline, has been developed. The possibility of determination of amino acid esters in mixtures containing free amino acids has been demonstrated. It is noted that determination of glycine and histidine with the help of o-phthaldialdehyde has certain specificities associated with faster, compared to other amino acids, degradation of their derivatives. Optimal conditions for quantitative analysis of amino acids in solutions of higher than 10?5m concentration are recommended. The reproducibility of the determination was ±2%. |
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