小麦体细胞无性系SSR位点的遗传变异特性分析

研究结果表明:(1)小麦体细胞无性系SSR位点变异类型有:扩增片段迁移率的变大或变小、扩增片段缺失以及新的扩增片段;(2)变异特点为:变异频率与基因型有关,不同染色体组上的SSR位点变异频率不同,而不同无性系后代的SSR位点变异频率也不同;(3)同一SSR位点的变异类型在同一基因型的无性系后代中变异表现一致,在不同基因型无性系后代中的变异表现不同,有的SSR位点在无性系后代中表现出一致的变异,而有的则不一致。

Analysis of Genetic Variation Character on SSR Loci of Somaclones in Wheat (Triticum aestivum L.)

Research results indicated that the types of somaclonal variation on SSR loci in wheat consist of addition, deletion, absence of DNA fragments and newly amplified band; the frequency of somaclonal variation on SSR loci was related to donor genotypes, different frequency of somaclonal variation was found on SSR loci located on different chromosome groups; and also among different somaclonal progenies derived from the same donor genotypes and the differentones; the type of somaclonal variation was same on SSR loci in the somaclonal progenies derived from the same genotype, but different in the somaclonal progenies derived from different genotype; on some SSR loci, the type of somaclonal variation were same in the somaclonal progenies, and on other SSR loci, that were not the same.