Culture and Generic Identification of Trypanosomatids of Phytophagous Hemiptera in Brazil |
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| Authors: | CLIZETE SBRAVATE MARTA CAMPANER LUIS E. A. CAMARGO IVETE CONCHON MARTA M. G. TEIXEIRA E. PLESSMANN CAMARGO |
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| Affiliation: | Departamento de Parasitologia, Instituto de Ciéncias Biomédicas, Universidade de São Paulo, 05508, São Paulo, Brazil;Departamento de Fitopatologia, ESALQ, 13400, Piracicaba, São Paulo, Brazil |
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| Abstract: | Among the 372 phytophagous Hemiptera examined, 133 insects of 28 species (Coreidae 18, Pentatomidae 7, Pyrrhocoridae 2, Lygaeidae 1) were infected with trypanosomatids. Gut infections only were found in 68.4%, gut and salivary gland infections in 29.3% and salivary infections alone in 2.3%. Fifty-one cultures were isolated from 38 insects. Cultures were characterized by assay of certain ornithinc-arginine metabolism enzymes and by indirect immunofluorescence against monoclonal antibodies specific for Phytomonas spp. Ten cultures were identified as either Crithidia or Leptomonas. Twenty-one promastigote cultures had an enzyme pattern hitherto recorded only for Leishmania and 16 cultures were identified as Phytomonas. |
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| Keywords: | Enzymes monoclonal antibodies Phytomonas taxonomy |
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