An RFLP map of diploid Hordeum bulbosum L. and comparison with maps of barley (H. vulgare L.) and wheat (Triticum aestivum L.) |
| |
Authors: | H Salvo-Garrido D A Laurie B Jaffé J W Snape |
| |
Institution: | (1) John Innes Centre, Norwich Research Park, Colney Lane, Norwich NR4 7UH, UK e-mail: john.snape@bbsrc.ac.uk, GB |
| |
Abstract: | This paper describes the first extensive genetic map of Hordeum bulbosum, the closest wild relative of cultivated barley. H. bulbosum is valuable for haploid production in barley breeding, and because of desirable agronomic characteristics, it also has potential
for trait introgression into barley. A H. bulbosum map will assist introgression and provide a basis for the identification of QTLs for crossability with barley and other potentially
useful genes. The present study used a population of 111 individuals from a PB1×PB11 cross to develop a genetic linkage map
of diploid H. bulbosum (2n=2x=14) based on barley, wheat and other ”anchor” cereal RFLP markers previously mapped in other species. Because of the cross-pollinating
and highly polymorphic nature of H. bulbosum, up to four alleles showed segregation at any one locus, and five different segregation types were found. This enabled maps
to be developed for the PB1 and PB11 parents, as well as a combined map. In total, 136 RFLP loci were mapped with a marker
coverage of 621 cM. The markers were generally colinear with barley but H. bulbosum had less recombination in the centromeric regions and similar or more in the distal regions. Cytological studies on pollen
mother cells at metaphase-I showed marked distal localization of chiasmata and a frequency consistent with the genetic map
length. This study showed that H. bulbosum was highly polymorphic, making it suitable for trait analysis and supplementing maps of barley.
Received: 20 November 2000 / Accepted: 5 January 2001 |
| |
Keywords: | Hordeum bulbosum Hordeum vulgare RFLP Genetic maps Comparative mapping |
本文献已被 SpringerLink 等数据库收录! |
|