Organization of collagen bundles during tendon healing in rats treated with L-NAME |
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Authors: | Tatiana Carla Tomiosso Wilson Romero Nakagaki Laurecir Gomes Stephen Hyslop Edson Rosa Pimentel |
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Institution: | (1) Department of Anatomy, Cell Biology, Physiology and Biophysics - Institute of Biology, State University of Campinas - UNICAMP, CP 6109, 13083–863 Campinas, SP, Brazil;(2) Department of Pharmacology, School of Medical Sciences, State University of Campinas - UNICAMP, CP 6109, Campinas, 13083-863, SP, Brazil;(3) Interdisciplinary Department - PURO, Fluminense Federal University - UFF, Rio das Ostras, RJ, Brazil |
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Abstract: | The Achilles tendon can support high tension forces and may experience lesions. The damaged tissue does not regenerate completely,
with the organization and mechanical properties of the repaired tendon being inferior to those of a healthy tendon. Nitric
oxide (NO) plays an important role in wound repair. We have examined the structural reorganization and repair in Achilles
tendon after injury in rats treated with the NO synthase inhibitor L-NAME. The right Achilles tendon of male Wistar rats was
partially transected. One group of rats was treated with L-NAME (~300 mg/kg per day, given in drinking water) for 4 days prior
to tendon sectioning and throughout the post-operative period. Control rats received water without L-NAME. The tendons were
excised at 7, 14, and 21 days post-injury and used to quantify hydroxyproline and for mechanical tests. Tendons were also
processed for histomorphological analysis by polarized light microscopy, which showed that the collagen fibers were disorganized
by day 7 in non-treated and L-NAME-treated rats. In non-treated rats, the organization of the extracellular matrix was more
homogeneous by days 14 and 21 compared with day 7, although this homogeneity was less than that in normal tendon. In contrast,
in injured tendons from L-NAME-treated rats, the collagen fibers were still disorganized on day 21. Tendons from treated rats
had more hydroxyproline but lower mechanical properties compared with those from non-treated rats. Thus, NO modulates tendon
healing, with a reduction in NO biosynthesis delaying reorganization of the extracellular matrix, especially collagen.
T.C.T. and W.R.N were supported by studentships from CAPES, and S.H. was supported by a research fellowship from Conselho
Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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Keywords: | Biomechanics Collagen Tendon L-NAME Nitric oxide Rat (Wistar male) |
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