High hydrostatic pressure approach proves RNA catalytic activity without magnesium |
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Authors: | Fedoruk-Wyszomirska Agnieszka Wyszko Eliza Giel-Pietraszuk Małgorzata Barciszewska Mirosława Z Barciszewski Jan |
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Affiliation: | Institute of Bioorganic Chemistry, Polish Academy of Sciences, Noskowskiego 12/14, 61-704 Poznan, Poland. |
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Abstract: | High hydrostatic pressure (HHP) technique was used to evaluate a mechanism of RNA hydrolysis with RNA. We showed that hammerhead ribozyme specifically cleaves RNA substrate at HHP in the absence of Mg(2+). A deoxyribozyme "10-23" was active in the same conditions. These results pointed out that the hydrolytic activity of nucleic acid depends on proper tertiary structure of a complex with a substrate. They prove that magnesium ion is not directly involved in catalysis process. On that basis we show the mechanism of RNA hydrolysis catalyzed with nucleic acids at HHP. |
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