The ionic hemolymph composition of the terrestrial isopod Porcellio scaber Latr. during molt |
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Authors: | A. Ziegler F. H. E. Scholz |
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Affiliation: | Sektion Elektronenmikroskopie, Universit?t Ulm, D-89069 Ulm, Germany Tel.:+49-731/502 3444, Fax:+49-731/502 3383, e-mail: andreas.ziegler@medizin.uni-ulm.de, DE Institut für Tierphysiologie, Freie Universit?t Berlin, D-12165 Berlin, Germany, DE
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Abstract: | We analyzed the ionic composition of the hemolymph of Porcellio scaber in four different stages of the molt cycle using capillary electrophoresis and calcium selective mini- and microelectrodes. The main ions in the hemolymph were K+, Ca2+, Na+, Mg+, and Cl−. The values for total calcium obtained by means of capillary electrophoresis and calcium selective minielectrodes did not differ significantly from each other. In situ measurements of the free Ca2+ concentration ([Ca2+]) by means of calcium-selective microelectrodes indicated that Ca2+ is not bound in the hemolymph. During molt the [Ca2+] is significantly larger than during intermolt. The [Ca2+] increased by 13%, 19% and 18% during premolt, intramolt, and postmolt, respectively. The concentration of the other cations and of Cl− decreased significantly between premolt and intramolt. Thus, the rise of the [Ca2+] in the hemolymph is not due to a general increase in all ions, but rather to the resorption of cuticular calcium. Furthermore, the results suggest that K+, Na+, Mg+, and Cl−are extruded from the hemolymph during and/or after posterior ecdysis. Accepted: 5 August 1997 |
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Keywords: | Calcium Capillary electrophoresis Hemolymph Isopod Molting |
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