Optimization of production of caffeine demethylase by <Emphasis Type="Italic">Pseudomonas</Emphasis> sp. in a bioreactor |
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Authors: | Sathyanarayana N Gummadi Swati Sucharita Dash Santhosh Devarai |
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Institution: | (1) Department of Biotechnology, Indian Institute of Technology-Madras, Chennai, 600 036, India |
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Abstract: | The effect of pH, aeration rate, and agitation rate on specific productivity of caffeine demethylase from Pseudomonas sp. was studied in a bioreactor. Maximum specific productivity of caffeine demethylase of 2,214 U g cell dry weight−1 h−1 was obtained at 0.27 vvm, 700 rpm, and pH 7.0. Under these conditions, volumetric oxygen transfer coefficient was 74.2 h−1, indicating that caffeine demethylase production by Pseudomonas sp. was highly oxygen-dependent. Different metabolite formation at different agitation and aeration rates can be used as
a strategy for recovery of pharmaceutically important metabolites from caffeine by manipulation of conditions in a bacterial
culture. This is the first report on production of high levels of caffeine demethylase in bioreactors. |
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Keywords: | Aeration rate Agitation rate Bioreactor Caffeine degradation Caffeine demethylase Pseudomonas sp |
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