叶下珠药材HPLC指纹图谱的比较研究

采用反相高效液相色谱法,对叶下珠药材不同产地、不同采收期、不同部位指纹图谱进行测定和比较分析,并与中成药叶下珠胶囊的HPLC指纹图谱进行了比较研究。结果显示:(1)建立了叶下珠药材的HPLC特征指纹图谱,标定18个共有峰,利用对照品指认4个峰;11批(来源地不同)叶下珠样品的HPLC图谱相似度(相合系数,均值)在0.89~0.99之间。(2)7批不同采收期的叶下珠药材的HPLC指纹图谱相似度在0.94以上,各共有峰的峰面积大多随生长期而增加,至10月5日达到最高,建议叶下珠药材应于每年的10月上旬进行采收。(3)不同部位叶下珠药材HPLC指纹图谱相似度分析发现,叶、果的相似度较高(0.98~0.99),根、茎的相似度较低(0.86~0.87),说明根、茎中各成分含量较低,建议采收叶下珠药材的地上部分即可。(4)比较叶下珠胶囊和叶下珠药材的指纹图谱,发现二者的化学成分非常相似,但峰面积差异较大,其差异可能是由于加工过程所致。该研究所建立的HPLC指纹图谱分析方法简便、重现性好,可用于叶下珠药材及中成药的鉴定与质量评价。

Comparative Study on HPLC Fingerprint of Phyllanthus urinaria

The HPLC fingerprint of Phyllanthus urinaria from different habitats, different harvest times, different parts was determined and compared by RP HPLC. The comparative study was done with the HPLC fingerprint of Yexiazhu Capsules also. The representative HPLC fingerprint of P. urinaria was established. The results showed: (1) There were 18 mutual peaks, four of which were identified by reference substance. The similarity of 11 samples from different habitats was in the range of 0.89 to 0.99. (2) The similarity of 7 samples from different harvest times was greater than 0.94. The peak area of most mutual peaks increased with growth period, the highest was in 5 October. It was suggested the best harvest time was on early October annually. (3) The similarity of samples from different parts was analyzed and evaluated. The similarity of leaf and fruit was higher in the range of 0.98 to 0.99, and the similarity of root and stem was in the range of 0.86 to 0.87. Lower content of components in root and stem brought about the difference in similarity. It was suggested only collecting the aerial parts. (4) The chemical constituents of Yexiazhu Capsules were nearly consistent with that of reference herb of P. urinaria, but the peak area of some peaks varied very much because of processing of Yexiazhu Capsules. So, this method is simple, accurate, and reproducible, which can be used for the quality control and evaluation of P. urinaria and its Chinese proprietary medicines.