| 常压室温等离子体诱变扭脱甲基杆菌AM1高产吡咯喹啉醌 |
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| 引用本文： | 李慧芝,康振,李江华,周景文,堵国成.常压室温等离子体诱变扭脱甲基杆菌AM1高产吡咯喹啉醌[J].生物工程学报,2016,32(8):1145-1149. |
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| 作者姓名： | 李慧芝 康振 李江华 周景文 堵国成 |
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| 作者单位： | 1 江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2 江南大学 生物工程学院，江苏 无锡 214122,1 江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2 江南大学 生物工程学院，江苏 无锡 214122,1 江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2 江南大学 生物工程学院，江苏 无锡 214122,1 江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2 江南大学 生物工程学院，江苏 无锡 214122,1 江南大学 工业生物技术教育部重点实验室，江苏 无锡 214122；2 江南大学 生物工程学院，江苏 无锡 214122 |
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| 基金项目： | 国家高技术研究发展计划 (863计划) (No. 2012AA022103)，国家重点基础研究发展计划 (973计划) (No. 2014CB745100)，新世纪优秀人才支持计划 (No. NCET-12-0876)资助。 |
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| 摘 要： | 吡咯喹啉醌(Pyrroloquinoline quinone,PQQ)作为一种新型的氧化还原酶辅酶,在医药和食品等领域有广阔的应用前景。为改善扭脱甲基杆菌Methylobacterium extorquens AM1 PQQ生产性能,采用常压室温等离子体(Atmospheric and room temperature plasma,ARTP)进行诱变,结合高通量快速筛选方法,得到以PQQ产量为指标的正向突变株。ARTP诱变的菌株正突变率为31.6%,筛选得到的较优正突变株M.extorquens AM1(E-F3),PQQ产量达到54.0 mg/L,是出发菌株的近3倍。系统的高通量方法筛选ARTP诱变菌为后续进一步提高M.extorquens AM1菌株PQQ的产量奠定了基础,亦为改善菌株生产性能提供了新思路。
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| 关 键 词： | 常压室温等离子体，吡咯喹啉醌，高通量筛选，正向突变 |
| 收稿时间： | 2015/12/9 0:00:00 |
Mutagenesis of Methylobacterium extorquens AM1 for increasing pyrroloquinoline quinone production by atmospheric and room temperature plasma |
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| Huizhi Li,Zhen Kang,Jianghua Li,Jingwen Zhou and Guocheng Du.Mutagenesis of Methylobacterium extorquens AM1 for increasing pyrroloquinoline quinone production by atmospheric and room temperature plasma[J].Chinese Journal of Biotechnology,2016,32(8):1145-1149. |
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| Authors: | Huizhi Li Zhen Kang Jianghua Li Jingwen Zhou and Guocheng Du |
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| Institution: | 1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China,1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China,1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China,1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China and 1 Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, Jiangsu, China; 2 School of Biotechnology, Jiangnan University, Wuxi 214122, Jiangsu, China |
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| Abstract: | As a novel cofactor of oxidoreductase, pyrroloquinoline quinone (PQQ) has a great potential of application in medicine, food industries. In order to improve the efficiency of the PQQ production by Methylobacterium extorquens AM1, the strain was treated by atmospheric and room temperature plasma (ARTP). Positive mutants with changes in PQQ yield were obtained based on a high-throughput screening approach. After ARTP treatment, analysis data show that the positive mutation rate was 31.6%. Furthermore, we obtained an excellent positive mutant M. extorquens AM1 (E-F3) with the yield of 54.0 mg/L PQQ, which was approximately 3 times as much compared with that of the wild-type strain. The robust high-throughput screening method for mutagenesis by ARTP improves PQQ production. In addition, this method also provides a new strategy for further strain improvement. |
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| Keywords: | atmospheric and room temperature plasma pyrroloquinoline quinone high-throughput screening positive mutation |
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