小黑麦抗真菌蛋白组分的分离纯化和性质研究

以木霉为指示菌，小黑麦中饲237种子中的蛋白提取物经过分离纯化后，得到了3种主要的抗真菌蛋白组分，经酶活检测鉴定，分别是分子量为30.5 kD的ClassⅡ型几丁质酶，两种分子量为51kD和23 kD的β-1,3-葡聚糖酶。其中几丁质酶的最适反应pH为6.0，最适反应温度为37℃，测定的N末端氨基酸序列与大麦几丁质酶的有很高的同源性。在一定条件下，这3种蛋白组分都有较强的抗木霉活性，并且有明显的协同作用，同时它们对离体易感小麦叶片上白粉菌有很好的生长抑制作用。

Purification and Characterization of Antifungal Proteins in Triticale Seed

Using Trichoderma as an indicative fungus, three antifungal proteins in Triticale Zhongsi 237 seed were purified and characterized. These protein components were considered to be a new Class II chitinase and two kinds of beta-1, 3-glucanases. Chitinase molecular mass was 30.5 kD and enzyme activity was maximal at pH 6.0 and 37 degrees C. Two beta-glucanases molecular masses were 51 kD and 23 kD. N-terminal amino acid sequences of Triticale chitinase share high homology with barley chitinase. In some conditions, the chitinase and beta-glucanases all had strong antifungal activity and were able to inhibit Trichoderma growth synergistically. Moreover, the chitinase and beta-1, 3-glucanases were able to inhibit powdery mildew growth on detached susceptible wheat leaves.