Optimizing conditions for cryopreservation of an insect cell line |
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Authors: | A.E. Heacox R.A. Leopold |
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Affiliation: | 1. Department of Zoology, North Dakota State University, Fargo, USA;2. U.S. Department of Agriculture, Agricultural Research Service, Metabolism and Radiation Research Laboratory, Fargo, North Dakota 58105 USA |
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Abstract: | A cell line (UM-BGE-2) derived from embryos of the cockroach Blattella germanica was frozen to ?196 °C under a variety of conditions and cell viability was assayed after warming. It was found that cell viability was affected by the cooling rate, the warming rate, the controlled cooling endpoint temperature, and the type and concentration of cryoprotectant. The best survival for cells suspended in Grace's tissue culture medium containing 1 M Me2SO was obtained when cells were cooled at 1 °C/ min to at least ?90 °C before being placed in liquid nitrogen and warmed at more than 900 °C/min. Cultures initiated from these frozen cells produce typical growth curves and appear normal after several passages. |
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Keywords: | To whom reprint requests should be addressed at U.S. Department of Agriculture Agricultural Research Service Metabolism and Radiation Research Laboratory State University Station Fargo N.D. 58105. |
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