Concanavalin A induces germinal vesicle breakdown in Barnea candida (Mollusca,Pelecypoda) oocytes |
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Authors: | Louise Dufresne-Dubé Christine Métivier François Dubé Pierre Guerrier |
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Institution: | 1. Station Biologique, 29211 Roscoff, France;2. Département Sciences Biologiques, Université de Montréal, C.P. 6128, Suc. A, Montréal, Québec, H3C 3J7, Canada |
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Abstract: | Treatment of Barnea candida oocytes with 5 μg/ml Con A or above elicits germinal vesicle breakdown (GVBD), the timing for this event being dose dependent. At 100 μg/ml, GVBD occurs within 20 to 30 min, a lag time corresponding to that observed after fertilization. Con A-induced GVBD requires the presence of 2 mM external calcium during all the treatment period while at 10 mM external Ca2+, the calcium-dependent period is slightly reduced. It is sensitive to low pH Na-acetate sea water, 50 μM trifluoperazine, 20μM D-600 or 2,4-dinitrophenol, as well as to 10 μg/ml cytochalasin B. A straightforward interpretation of these data would be that Con A-induced maturation is sustained by an energy-requiring effector mechanism involving intracellular contractile proteins. |
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