Developmental uncoupling between blastoderm and yolk cell in the embryo of the teleost Fundulus |
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Authors: | Charles B. Kimmel David C. Spray M.V.L. Bennett |
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Affiliation: | Marine Biological Laboratory, Woods Hole, Massachusetts 02543 USA |
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Abstract: | During cleavage and blastula stages of embryos of the teleost Fundulus heteroclitus all of the cells are both electotonically coupled and dye coupled to one another, as determined by microelectrode impalements and spread of Lucifer Yellow. At about the time that gastrulation begins we observed a specific loss of junctional coupling between the yolk cell and cells of the blastoderm. Passage of Lucifer Yellow between the yolk cell and blastoderm was reduced at stage 12 (late blastula), and not detected at stage 13 and thereafter, although cells of the blastoderm remain dye coupled to one another through gastrula stages. Also, junctional electrical coupling between the yolk cell and blastoderm became substantially reduced at stage 13 and thereafter. The loss of coupling at this specific cell apposition and time and the large size of the yolk cell may prove useful in analyzing the underlying cellular mechanisms. |
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Keywords: | To whom reprint requests should be addressed at the Institute for Neuroscience University of Oregon Eugene Oregon 97403. |
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