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The biosynthesis of a mannolipid that contains a polar metabolite of 15-14C-retinol
Authors:L De Luca  G Rosso  G Wolf
Affiliation:1. Department of Biomolecular Sciences, Max Planck Institute of Colloids and Interfaces, 14476 Potsdam, Germany;2. Institute of Chemistry and Biochemistry, Freie Universität Berlin, 14195 Berlin, Germany;3. Institute for Glycomics, Griffith University, Gold Coast Campus, QLD, 4222, Australia;4. ARC Centre for Nanoscale BioPhotonics, Australia;1. Complex Carbohydrate Research Center, University of Georgia, Athens, GA 30602, USA;2. Department of Chemistry, University of Georgia, Athens, GA 30602, USA;3. Chemical Biology and Drug Discovery, Utrecht University, 3584 CG Utrecht, The Netherlands;4. Utrecht Institute for Pharmaceutical Sciences, Utrecht University, 3584 CG Utrecht, The Netherlands;5. Bijvoet Center for Biomolecular Research, Utrecht University, 3584 CH Utrecht, The Netherlands;1. Japan Science and Technology Agency (JST), ERATO, Ito Glycotrilogy Project, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan;2. Synthetic Cellular Chemistry Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
Abstract:A mannolipid synthesized by a membrane-rich fraction from rat liver in the presence of 15-14C-retinol and GDP-mannose-14C contained a polar metabolite of 14C-retinol, as well as 14C-mannose. Retinol stimulated the in vitro incorporation of 14C-mannose from GDP-mannose-14C into the mannolipid and glycoproteins.
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