首页 | 本学科首页   官方微博 | 高级检索  
   检索      


Cloning and heterologous expression of a novel insecticidal gene (tccC1) from Xenorhabdus nematophilus strain
Authors:Joo Lee Pom  Ahn Ji-Young  Kim Yang-Hoon  Wook Kim Seung  Kim Ji-Yeon  Park Jae-Sung  Lee Jeewon
Institution:Department of Chemical and Biological Engineering, Korea University, Anam-Dong 5-1, Sungbuk-Ku, Seoul 136-701, South Korea.
Abstract:We have identified and cloned a novel toxin gene (tccC1/xptB1) from Xenorhabdus nematophilus strain isolated from Korea-specific entomophagous nematode Steinernema glaseri MK. The DNA sequence of cloned toxin gene (3048 bp) has an open reading frame encoding 1016 amino acids with a predicted molecular mass of 111058 Da. The toxin sequence shares 50-96% identical amino acid residues with the previously reported tccC1 cloned from X. nematophilus, Photorhabdus luminescens W14 P. luminescens TTO1, and Yersinia pestis CO92. The toxin gene was successfully expressed in Escherichia coli, and the recombinant toxin protein caused a rapid cessation in mortality of Galleria mellonella larvae (80% death of larvae within 2 days). Conclusively, the heterologous expression of the novel gene tccC1 cloned into E. coli plasmid vector produced recombinant toxin with high insecticidal activity.
Keywords:Toxin gene (tccC1/XptB1)  Xenorhabdus nematophilus  Heterologous expression  High insecticidal activity
本文献已被 ScienceDirect PubMed 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号