双歧杆菌QJ405、乳杆菌QJ405在试管内对肠道致病菌抑杀效果的初步报告

目的 观察双歧杆菌ＱＪ４０５和乳杆菌ＱＪ４０５在试管内对肠道致病的抑菌和杀菌效果。方法 抑菌实验：实验组，双歧杆菌ＱＪ４０５和乳杆菌ＱＪ４０５分别与各致病菌共同培养；对照组，实验组中的细菌各自单独培养。然后观察一定间隔时间（每２ｈ或４ｈ）细菌的数量变化，并绘制生长曲线。杀菌实验：将２种菌的培养上清液定量滴在已均匀涂布定量致病菌的平板表面，然后观察滴过上清液的区域内是否有细菌生长。结果：４种肠道致病菌和２株标准菌株（ＡＴＣＣ２５２９２２，ＡＴＣＣ２５９２３）的生长明显受到抑制。乳杆菌ＱＪ４０５的代谢产物有杀菌作用。结论 双歧杆菌ＱＪ４０５和乳杆菌ＱＪ４０５具有益生菌的性质。

PRELIMINARY REPORT OF EFFECT ON INHIBITION ANDDISINFECTION OF ENTEROPATHOGENS BY BIFIDOBACTERIUM QJ405 AND LACTOBCILLUS QJ405IN VITRO

Objective:To observe the effect of inhibition and disinfection of Enteropathogens by Bifidobactium QJ405 and Lactobacillus QJ405 in vitro.Methods:The inhibiting test was performed on two groups:One was the testing group in which Bifidobacterium QJ405 and Lactobacillus QJ405 were cutured dlone respectively.The change of number of the bacteria had been observed at an inter of two of four hours,and the curve of bacterial grouth bad been drawn.In the disinfecting test,the metabolite of Bifidobecterium QJ405 and Lactobacillus QJ405 quantitatively inoculated respectively on the EMB agqar medium's surface which was spreaded by the quantative enteropathogens in advance,and the growing state of these bacteria was observed in the inoculated region at the following step.Results:It is obvious in our test that the grouth of hour strains of Enteropathogens and the two standard strains(ATCC2592,ATCC25923)were inhibited by Bifidobacterium QJ405 and Lactobacillus QJ405 and the merabolite of Lactobacillus QJ405 could destroy the Eneropthogens.Conclution:Bifidobacterium QJ405 and Lactobacillus QJ405 have some antibiotic effects as the probiotics.