Integration of 18O labeling and solution isoelectric focusing in a shotgun analysis of mitochondrial proteins |
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Authors: | Wang Jinshan Gutierrez Peter Edwards Nathan Fenselau Catherine |
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Affiliation: | Department of Chemistry and Biochemistry, University of Maryland, College Park, Maryland 20742, USA. |
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Abstract: | Forward and reverse (18)O labeling are integrated with solution isoelectric focusing and capillary LC-tandem mass spectrometry to evaluate a new strategy for quantitative proteomics and to study abundance changes in mitochondrial proteins associated with drug resistance in MCF-7 human cancer cells. Galectin-3 binding protein, which is involved in apoptosis, was detected only in the resistant cell line, as a result of reverse labeling. Among 278 proteins identified, 12 were detected with abundances altered at least 2-fold. |
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