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Capillary electrophoretic separation and quantification of flavone-O- and C-glycosides in Achillea setacea W. et K
Authors:Marchart Elke  Kopp Brigitte
Institution:Institute of Pharmacognosy, PharmaCenterVienna, University of Vienna, Althanstrasse 14, 1090 Vienna, Austria.
Abstract:A simple method for the rapid separation and quantification of flavone-O- and C-glycosides in A. setacea W. et K. by capillary zone electrophoresis (CZE) with UV detection is described. Using 25 mM sodium borate with 20% (v/v) of methanol (pH 9.3) as running buffer sufficient separation of the analytes was achieved within 19 min. For the quantitative determination isorhamnetin-3-O-rutinoside was used as internal standard. The method was successfully applied to a rapid characterisation of the flavonoid complex and a precise quantification of the single and total amount of the flavonoids in different samples of A. setacea.
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