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Microinjection of RNA polymerase II corrects the temperature-sensitive defect of tsAF8 cells |
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| Authors: | Dieter E Waechter Carlo Avignolo Erwin Freund C Mark Riggenbach W Edward Mercer Peter M McGuire Renato Baserga |
| Institution: | (1) Department of Pathology and Fels Research Institute, Temple University Medical School, 19140 Philadelphia, PA, USA;(2) Department of Biochemistry and Molecular Biology, University of Florida, 32610 Gainesville, FL, USA;(3) Present address: Friedrich Miescher Inst., P.O. Box 273, CH-4002 Basel, Switzerland |
| Abstract: | Summary tsAF8 cells area temperature-sensitive (ts) mutant of BHK cells that arrest in the G1 phase of the cell cycle at the non-permissive temperature of 40.6 °C. Previous reports had suggesed that the temperature-sensitivity of these cells was based on a defect in either the synthesis, assembly or turnover of RNA polymerase II. We now show that the direct microinjection of purified RNA polymerase 11 into nuclei of tsAF8 cells corrects the ts defect and allows these cells to enter the S phase of the cell cycle. |
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