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| 韭不同倍性及其非整倍体的ITS序列变异研究 | |
| 引用本文： | 戴波,顾红雅,瞿礼嘉,虞泓.韭不同倍性及其非整倍体的ITS序列变异研究[J].云南植物研究,2003,25(4):483-490. |
| 作者姓名： | 戴波 顾红雅 瞿礼嘉 虞泓 |
| 作者单位： | 1. 云南大学发展研究院,云南,昆明,650091 2. 北京大学蛋白质工程和植物基因工程国家重点实验室,北京,100871 3. 云南大学生命科学学院生态遗传学实验室,云南,昆明,650091 |
| 基金项目： | 云南省“九五”科技攻关项目 (95A - 5 )资助，云南大学植物学博士点基金资助，云南大学省级生物技术人才培养基地项目资助 |
| 摘 要： | 对不同倍性及其非整倍体韭Allium tuberosum和野韭Allium ramosum核糖体DNA的内部转录区(ITS)进行了PCR扩增和克隆，并测定10个ITS1和ITS2的序列，用ITS1 ITS2的序列探讨Allium tuberosum和Allium ramosum的系统发育关系。研究表明，Allium tuberosum与Alli-um ramosum种间的平均Kimura遗传距离仅为0．0146，二者是一对近缘种。虽然韭经历了长期的人工选择和自然选择，但与野韭的分化仍然较小。韭不同倍性及其非整倍体之间，其ITS序列亦无明显差异。 |
| 关 键 词： | 韭 倍性 非整倍体 ITS序列 变异 葱属 |
| 文章编号： | 0253-2700(2003)04-0483-08 |
| 修稿时间： | 2002年9月13日 |
Studies on Variation of ITS Sequences with Different Ploidy and Its Aneuploid in Allium tuberosum |
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| DAI Bo ,GU Hong-Ya ,QU Li-Jia ,YU Hong.Studies on Variation of ITS Sequences with Different Ploidy and Its Aneuploid in Allium tuberosum[J].Acta Botanica Yunnanica,2003,25(4):483-490. | |
| Authors: | DAI Bo GU Hong-Ya QU Li-Jia YU Hong |
| Institution: | DAI Bo 1,GU Hong-Ya 3,QU Li-Jia 3,YU Hong 2 |
| Abstract: | The ITS regions of 2 closely related species Allium tuberosum Rottler. ex Spreng.and Alllium ramosum L. in Allium Sect. Rhiziridium G. Don were amplified, cloned and sequenced. The genetic relationships among 10 materials of diploid,triploid,tetraploid and aneuploid in Allium tuberosum and Alllium ramosum were reconstructed based on ITS1+ITS2 sequences. The results demonstrated that the average Kimura pairwise distance between Allium tuberosum and Allium ramosum was 0.0146.They are a pair of closely related species. Although it was gone through evolution by artificial selection and natural selection, Allium tuberosum kept little differentiation from Allium ramosum .Sequences of ITS region show that there are no evident differentiation among the various diploid,triploid,tetraploid and aneuploid of Allium tuberosum . |
| Keywords: | Allium tuberosum Allium ramosum ITS sequence Polyploidy Aneuploid |
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