| 基于CATS法的江豚SNPs位点筛选 |
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| 引用本文： | 李树珍,万慧荣,杨光. 基于CATS法的江豚SNPs位点筛选[J]. 动物学报, 2008, 54(4) |
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| 作者姓名： | 李树珍 万慧荣 杨光 |
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| 作者单位： | 南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室,南京 210097 |
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| 基金项目： | 国家自然科学基金，教育部跨世纪优秀人才培养计划，高等学校博士学科点专项科研项目，江苏省高校重大基础研究计划支持项目 |
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| 摘 要： | 通过比较锚定序列示踪(Comparative Anchor Tagged Sequences, CATS)法,选择人、小鼠及其它哺乳动物中已知的5对CATS引物,通过PCR反应从江豚(Neophocaena phocaenoides)基因组中扩增出相应的DNA片段,结合变性高效液相色谱(Denaturing High Performance Liquid Chromatography, DHPLC)和DNA直接测序等技术,进行江豚单核苷酸多态性(Single nucleotide polymorphisms, SNPs)位点的筛选.通过不同个体同源序列的比对分析,发现其中的4个DNA片段具有多态性,共检测出7个SNPs位点,即大约每370 bp出现1个SNPs.本研究为进一步利用CATS法获得江豚及其它野生动物种群的SNPs位点并进行种群遗传学分析奠定了基础
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| 关 键 词： | 江豚 |
Single nucleotide polymorphisms of the finless porpoise Neophocaena phocaenoides revealed by using comparative anchor tagged sequences |
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| LI Shu-Zhen,WAN Hui-Rong,YANG Guang. Single nucleotide polymorphisms of the finless porpoise Neophocaena phocaenoides revealed by using comparative anchor tagged sequences[J]. Acta Zoologica Sinica, 2008, 54(4) |
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| Authors: | LI Shu-Zhen WAN Hui-Rong YANG Guang |
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| Affiliation: | LI Shu-Zhen,WAN Hui-Rong,YANG Guang Jiangsu Key Laboratory for Biodiversity , Biotechnology,College of Life Sciences,Nanjing Normal University,Nanjing 210097,China |
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| Abstract: | Comparative Anchor Tagged Sequences (CATS) were used to screen novel single nucleotide polymorphisms (SNPs) in the finless porpoise Neophocaena phocaenoides. Five loci were screened by using Polymerase Chain Reaction (PCR), Denaturing High Performance Liquid Chromatography (DHPLC), and direct sequencing. All the primer sets were based upon gene sequences from human, mouse and some other mammals. Seven SNPs were identified from four of the five sequenced regions, yielding a SNP on average every 370 base pair... |
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| Keywords: | SNPs CATS DHPLC |
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