Concentration of dilute solutions of macromolecules by electrophoresis |
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| Authors: | R F Atmeh |
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| Affiliation: | Department of Allied Health Sciences, University of Science and Technology, Irbid, Jordan. |
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| Abstract: | An electrophoresis apparatus which is used for concentrating micrograms of macromolecules from solutions as large as 250 ml is described. The recoveries were greater than 91% with three different macromolecules tested (28 to 360 kDa). Solutions with volumes in the range of 35 ml were concentrated 70-fold in less than 90 min to a final volume of 0.5 ml. Larger volumes in the range of 250 ml were concentrated 227-fold in 16 h to a final volume of 1.1 ml. Sterile concentrates can be obtained if the apparatus is constructed under sterile conditions. |
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