Production of an antibody against guinea pig MIF. I. Specificity of the anti-MIF antibody. |
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| Authors: | K Onozaki S Haga K Miura M Ichikawa T Hashimoto |
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| Institution: | 1. Institute of Basic Medical Sciences, University of Tsukuba, Ibaraki-Ken, Japan;2. National Institute of Health, Shinagawa-Ku, Tokyo, Japan |
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| Abstract: | An antibody was produced in rabbits against partially purified MIF which was released from the specifically stimulated lymphocytes of tuberculin-hypersensitive guinea pigs. The MIF used as an antigen was fractionated by polyacrylamide gel electrophoresis. The antibody thus prepared was then examined for its specificity for several lymphokines by affinity column chromatography. It was observed that the antibody column adsorbed MIF, but not the other three lymphokines, MCF, NCF, and SRF, indicating a keen specificity of the antibody against MIF. |
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