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Tethered agonist exposure in intact adhesion/class B2 GPCRs through intrinsic structural flexibility of the GAIN domain
Authors:Gerti Beliu  Steffen Altrichter  Ramon Guixà-González  Mareike Hemberger  Ina Brauer  Anne-Kristin Dahse  Nicole Scholz  Robert Wieduwild  Alexander Kuhlemann  Hossein Batebi  Florian Seufert  Guillermo Pérez-Hernández  Peter W Hildebrand  Markus Sauer  Tobias Langenhan
Institution:1. Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, Am Hubland, 97074 Würzburg, Germany;2. Rudolf Schönheimer Institute of Biochemistry, Division of General Biochemistry, Medical Faculty, Leipzig University, Johannisallee 30, 04103 Leipzig, Germany;3. Institute for Medical Physics and Biophysics, Medical Faculty, Leipzig University, Härtelstrasse 16–18, 04107 Leipzig, Germany;4. Laboratory of Biomolecular Research, Paul Scherrer Institute (PSI), 5232 Villigen PSI, Switzerland;5. Condensed Matter Theory Group, PSI, 5232 Villigen PSI, Switzerland;6. Institute of Medical Physics and Biophysics, Charité - Universitätsmedizin Berlin, Charitéplatz 1, 10117 Berlin, Germany;7. Berlin Institute of Health, 10178 Berlin, Germany
Abstract:
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  • Keywords:adhesion GPCR  GAIN domain  genetic code expansion  FRET microscopy  tethered agonism  dSTORM  super-resolution microscopy  bioorthogonal click labeling  auto-proteolysis
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