Quantification of biochemical compounds in Bauhinia Variegata Linn flower extract and its hepatoprotective effect |
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Authors: | Hina Gul Muhammad Awais Salina Saddick Yazeed Ahmed Falak Sher Khan Eilaf Ahmed Umara Afzal Syed Muhammad Zaigham Abbas Naqvi Muhammad Asghar Khan Muhammad Gulfraz Ghazala Kaukab Raja |
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Affiliation: | 1. University Institute of Biochemistry and Biotechnology (UIBB), PMAS-Arid Agriculture University, Rawalpindi, Pakistan;2. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, P.O. Box 80203, Jeddah 21589, Saudi Arabia;3. School of Medicine, University of Central Lancashire, Preston, United Kingdom;4. Department of Biotechnology, University of Sialkot, Pakistan;5. Department of Electrical and Mechanical Engineering, Henan Agricultural University, Zhengzhou 450002, China;6. National University of Medical Sciences, Rawalpindi, Pakistan;7. Department of Biochemistry, National University of Pakistan, Sialkot, Pakistan;8. Department of Nanoscience and Technology, National Center for Physics, Islamabad, Pakistan |
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Abstract: | Liver disorders may occur as a result of exposure to chemical compounds capable of inducing the oxidative stress and hepatic injuries. The aim of present study was to investigate the effects of flower extracts of B. Variegata for the treatment of liver injury induced by the CCl4. About 1 ml/kg body weight (b.w) of CCl4 was induced to experimental mice by intraperitoneal way for 14 days. The methanol and chloroform extracts (100, 200 and 300 mg/kg b.w) were administered to experimental animals for 14 days along with standard drug Silymarine (100 mg/kg b.w). The extracts alone showed no evidence of hepatic toxicity but animals exposed to CCl4 without the treatment with B. Variegata presented variations in levels of liver enzymes, antioxidant enzymes, proteins and blood cells as well as injuries in liver cells were also observed during histopathological study. However, after the treatments especially with 300 mg/kg b.w of methanol flower extracts levels of liver markers (ALT, AST and ALP), antioxidant enzymes and blood cells decreases and turned towards normal levels. Whereas level of total proteins and bilirubin was improved and damaged liver cells were repaired. The curative activity of flower extracts can be correlated to the higher potential of antioxidants and occurrence of Quercetin and some other organic compounds those were investigated from flower extracts of B. Variegata during HPLC and GC-MS analysis. The finding of this study supports the use of B. Variegata flower formulation in folk medicines. |
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Keywords: | Phytochemicals HPLC Antioxidants Hepatoprotective effect |
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