Potential cytotoxic and mutagenic effect of Pinus wallichiana,Daphne oleiodes and Bidens chinensis |
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Authors: | Nazish Khan Ibrar Khan Sadiq Azam Farhan Ahmad Hamid Ali Khan Asma Shah Midrar Ullah |
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Affiliation: | 1. Centre of Biotechnology and Microbiology, University of Peshawar, Pakistan;2. Department of Biotechnology, Bacha Khan University, Charsadda, Pakistan;3. Institute of Biological Sciences Sarhad University of Science and Information Technology, Peshawar, Pakistan;4. Department of Biotechnology, Women University Mardan, KPK, Pakistan;5. Department of Biotechnology Shaheed Benazir Bhutto University Sheringil, Dir Upper Khyber Pakhtunkhwa, Pakistan |
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Abstract: | The Pinus wallichiana, Daphne oleiodes and Bidens chinensis have a long history of being used traditionally for treatment of various types of disorders. Most of the uses have been without any scientific evidence and toxicological assessment. We evaluated the mutagenic and cytotoxic capabilities of various parts of P. wallichiana, D. oleoides and B. chinensis. Ames Salmonella mutagenicity assay determined the mutagenicity activity against TA 98 and TA 100 bacterial strains of Salmonella typhimurium without metabolic activator S9 system. The number of mutant colonies in negative control was considered as limit to determine the mutagenicity effects of every extract. Brine shrimps lethality bioassay was used to determine the cytotoxic capabilities of the selected plants. The P. wallichiana, D. oleiodes and B. chinensis did not showed any mutagenic activity both for frameshift mutation (TA98) and base-pair substitution (TA100) without S9 mixture. The crude methanolic extract of P. wallichiana stem showed moderate cytotoxicity (53.33%) at 1000 μg/ml with LD50 value 599.634. The D. oleoides fruit showed a toxicity of 60% at 1000 μg/ml with LD50 value 367.730. The B. chinensis (whole plant) showed lethality of 63.3% at 1000 μg/ml, with LD50 204.833. The absence of any mutagenic activity of crude extract of the tested plants in both bacteria strains, TA 98 and TA 100 without the S9 mix confirms the safety of these plants to the consumers. |
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Keywords: | Pinus wallichiana Daphne oleiodes Bidens chinensis Ames Salmonella mutagenicity assay Brine shrimps lethality bioassay |
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