Preparation and quality by design assisted (Qb-d) optimization of bioceramic loaded microspheres for periodontal delivery of doxycycline hyclate |
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| Authors: | Pooja Jain Abhinav Garg Uzma Farooq Amulya K. Panda Mohd. Aamir Mirza Ahmed Noureldeen Hadeer Darwish Zeenat Iqbal |
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| Affiliation: | 1. Department of Pharmaceutics, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India;2. Product Development Cell, National Institute of Immunology, New Delhi, India;3. Department of Biology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;4. Department of Biotechnology, College of Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
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| Abstract: | PLGA (Lactic- co-glycolic acid) coated chitosan microspheres loaded with hydroxyapatite and doxycycline hyclate complex were developed in the present study for periodontal delivery. A modified single emulsion method was adopted for the development of microspheres. Formulation was optimized on the basis of particle size, drug loading and encapsulation efficiency with the central composite design using 23 factorial design. Microspheres were optimized and electron microscopy revealed their spherical shape and porous nature. In-vitro study showed initial burst and then sustained release behavior of the formulation for 14 days. Further, in-vitro antibacterial study performed on E. coli (ATCC-25922) and S. aureus (ATCC-29213) revealed concentration dependent activity. Also, in-vitro cyto-toxicity assessment ensures biocompatibility of the formulation with the fibroblast’s cells. Overall, the quality by design assisted PLGA microspheres, demonstrated the desired attributes and were found suitable for periodontal drug delivery. |
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| Keywords: | Bioceramic Composite Quality by design Central composite design Microspheres |
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