Protective effect of vanillic acid in hydrogen peroxide-induced oxidative stress in D.Mel-2 cell line |
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| Authors: | Shagufta Taqvi Eijaz Ahmed Bhat Nasreena Sajjad Jamal S.M. Sabir Aleem Qureshi Irfan A. Rather Suriya Rehman |
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| Affiliation: | 1. Department of Biochemistry and Biotechnology, Annamalai University, Chidambaram, Tamil Nadu, India;2. Life Science Institute, Zhejiang University, Hangzhou, Zhejiang 310058, PR China;3. Department of Biochemistry, University of Kashmir, Srinagar 190006, India;4. Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah, Saudi Arabia;5. Centre of Excellence in Bionanoscience Research, King Abdulaziz University (KAU), Jeddah 21589, Saudi Arabia;6. Department of Environmental Engineering, College of Engineering, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;7. Department of Epidemic Disease Research, Institute for Research and Medical Consultations (IRMC), Imam Abdulrahman Bin Faisal University, 31441 Dammam, Saudi Arabia |
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| Abstract: | The overproduction of reactive oxygen species (ROS) causes oxidative stress, such as Hydrogen peroxide (H2O2). Acute oxidative stress is one of the main reasons for cell death. In this study, the antioxidant properties of vanillic acid- a polyphenolic compound was evaluated. Therefore, this study aims to check the effectiveness of vanillic acid in H2O2-induced oxidative stress in D. Mel-2 cell line. The efficacy was determined by biochemical tests to check the ROS production. The cytotoxicity of H2O2 and vanillic acid was checked by MTT assay. The DNA fragmentation was visualized by gel electrophoresis. Protein biomarkers of oxidative stress were analyzed by western blotting. The results depict a promising antioxidant effect of vanillic acid. The IC50 value of vanillic acid and H2O2 was found 250 μg/ml and 125 μg/ml, respectively. The catalase activity, SOF, GPx, and PC was seen less in H2O2 treated group compared with the control and vanillic acid treated group. However, the TBRAS activity was hight in H2O2 treated group. The effect of H2O2 on DNA fragmentation was high as compared with vanillic acid-treated cells. The protein expression of Hsp70, IL-6 and iNOS was seen significant in a vanillic acid-treated group as compared with H2O2 treated group. These results reinforce that at low concentration, vanillic acid could be used as an antioxidant agent in the food and pharmaceutical industries. |
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| Keywords: | DNA fragmentation Hygrogen peroxide Oxidative stress Pharmaceuticals Vanillic acid |
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