Frequency distribution and association of Fat-mass and obesity (FTO) gene SNP rs-9939609 variant with Diabetes Mellitus Type-II population of Hyderabad,Sindh, Pakistan |
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| Authors: | Farheen Shaikh Tazeen shah Norah Abdullah Bazekh Madkhali Ahmed Gaber Walaa F. Alsanie Sanum Ali Shafaq Ansari Muhammad Rafiq R.Z. Sayyed Nadir Ali Rind Khalid Hussain Rind Akhtar Hussain Shar Syed Mohammed Basheeruddin Asdaq |
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| Affiliation: | 1. Department of Biochemistry, Peoples University of Medical and Health Sciences for Women, Shaheed Benazir Abad, Sindh, Pakistan;2. Department of Physiology, Liaquat University of Medical and Health Sciences, Jamshoro, (LUMHS) Sindh, Pakistan;3. College of Nursing, Jazan University, Jazan, Saudi Arabia;4. Department of Biology, College of Science, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;5. Center of Biomedical Sciences Research (CBSR), Taif University, P.O. Box, 11099, Taif 21944, Saudi Arabia;6. Department of Clinical Laboratories Sciences, The Faculty of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia;7. Department of Anatomy, Basic Medical Sciences Institute (BMSI), Jinnah Postgraduate Medical Centre, Karachi, Pakistan;8. Department of Physiology, Muhammad Medical College, Mirpurkhas, Sindh, Pakistan;9. Institute of Biotechnology and Genetic Engineering, University of Sindh, Jamshoro 76080, Sindh, Pakistan;10. Asian PGPR Society for Sustainable Agriculture, Auburn University, Auburn, AL 36830, USA;11. Department of Molecular Biology and Genetics, Shaheed Benazir Bhutto University, Shaheed Benazirabad, Sindh, Pakistan;12. Department of Pharmacy Practice, College of Pharmacy, AlMaarefa University, Dariyah, 13713, Riyadh, Saudi Arabia |
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| Abstract: | Background/AimDiabetes Mellitus (DM) is one of the important public health issues worldwide. The Fat mass obesity (FTO) gene rs-9939609 variant identified single nucleotide polymorphism (SNP) with the T to A missense mutation, and has a strong association with T2DM. FTO gene is present on chromosome “16q12.2” comprising of nine exons. FTO gene rs-9939609 a variant is commonly found in the Pakistani Population. The purpose of the study was to alert the population about the rs-9939609 variant SNP, having a strong association with T2DM.Material and MethodsTotal of 190 participants were included in the present cross-sectional study. To collect the samples non-probability convenience technique was used. subjects were recruited and divided into three groups, normal healthy subjects, obese and T2DM. The patients were selected from the Medicine department Jamshoro/Hyderabad by filling the pre-designed proforma, as well as verbal and written consent taken from study participants. To analysed the data ANOVA Post hoc (Tukey-test) was applied for comparison among groups (P < 0.05) and “SNP-STAT” online software was used for frequencies.ResultsThe BMI, neck circumference, waist circumference and lipid profile, fasting blood sugar and HbA1c was found significant (p < 0.001) in both genders as compared to control. Homozygous and heterozygous distribution of allelic and genotyping frequency was found in study participants. 37.9 %T/A, 57.4% T/T, and A/A were 4.7%. The FTO gene rs-9939609 variant amplified and have an increased risk of developing T2DM in the Sindh population. Codominant model odd ratio of T/A showed 2.42 (CI)1.23–3.84, with significant p < 0.032.ConclusionThe present study concluded that the FTO gene SNP rs-9939609 variant was found in the population of Hyderabad, Sindh and having strong association with T2DM and obese individuals. Increase BMI, neck and waist circumference are the biomarkers of obesity and causative factors of T2DM. |
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| Keywords: | BMI Obesity SNP T2DM |
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