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Optimization of protease production from Bacillus halodurans under solid state fermentation using agrowastes |
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| Authors: | Chellapandian Balachandran Alagumalai Vishali Natarajan Arun Nagendran Kathirvelu Baskar Abeer Hashem Elsayed Fathi Abd_Allah |
| Institution: | 1. Department of Biotechnology, Thiagarajar College, Madurai 625 009, Tamil Nadu, India;2. National Centre of Excellence (MHRD), Thiagarajar College, Madurai 625 009, Tamil Nadu, India;3. Department of Zoology, Thiagarajar College, Madurai 625 009, Tamil Nadu, India;4. Department of Ecotoxicology, Ross Lifescience, Pvt., Ltd., Bhosari, Pune, India;5. Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia;6. Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza 12511, Egypt;7. Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia |
| Abstract: | Marine microbes are potential source for novel metabolites. They are efficient in producing these metabolites utilizing agrowastes. Protease is one of the enzymes which find wide industrial applications. In the present study, protease producing bacteria was isolated from marine sediments and the organism was identified as Bacillus halodurans. The organism was subjected to protease production under solid state fermentation (SSF) using different agrowastes as substrates. Among the substrates used, wheat bran yielded maximum quantity of protease. The fermentation process was carried out under different cultural conditions to optimize the parameters influencing the enzyme production. The results of the stain removal studies by the enzyme revealed the increased efficiency of the microbial enzyme than the commercial detergent. |
| Keywords: | Sold state fermentation Agrowastes Protease Stain removal |
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