Recombinant Human erythropoietin reduces viability of MCF-7 breast cancer cells from 3D culture without caspase activation |
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| Authors: | Hareth Y. ShujaaEdin Nagi A. AL-Haj Abdullah Rasedee Noorjahan Banu Alitheen Arifah Abdul Kadir Chee Wun How Heshu Sulaiman Rahman Al-Shwyeh Hussah Abdullah |
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| Affiliation: | 1. Institute of Bioscience, Universiti Putra Malaysia, Malaysia;2. Faculty of Medicine and Health Sciences, Sana’a University, Yemen;3. Faculty of Veterinary Medicine, Universiti Putra Malaysia, Malaysia;4. Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, Malaysia;5. School of Pharmacy, Monash University Malaysia, Bandar Sunway, Selangor, Malaysia;6. College of Medicine, University of Sulaimani, Kurdistan Region, Iraq;7. College of Science, Imam Abdul Rahman bin Faisal University, Saudi Arabia |
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| Abstract: | Recombinant human erythropoietin (rHuEPO) is the erythropoiesis-stimulating hormone that is being used concurrently with chemotherapeutic drugs in the treatment of anemia of cancer. The effect of rHuEPO on cancer cells in 3-dimensional (3D) cultures is not known. The objective of the study was to determine the effect of rHuEPO on the viability of MCF-7 breast cancer cells from 2-dimensional (2D) and 3D cell cultures. The monolayer MCF-7 cells from 2D culture and MCF-7 cell from 3D culture generated by ultra-low adhesive microplate technique, were treated with 0, 0.1, 10, 100 or 200 IU/mL rHuEPO for 24, 48 or 72 h. The effects of rHuEPO on MCF-7 cell viability and proliferation were determined using the (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT), neutral red retention time (NRRT), trypan blue exclusion assay (TBE), DNA fragmentation, acridine orange/propidium iodide staining (AO/PI) assays. The MCF-7 cells for 3D culture were also subjected to caspase assays and cell cycle analysis using flow cytometry. rHuEPO appeared to have greater effect at lowering the viability of MCF-7 cells from 3D than 2D cultures.rHuEPO significantly (p < 0.05) decreased viability and down-regulated the caspase activities of 3D MCF-7 cells in dose- and time-dependent manner. The cell cycle analysis showed that rHuEPO caused MCF-7 cells to enter the subG0/G1 phase. Thus, the study suggests that rHuEPO has a cytostatic effect on the MCF-7 breast cancer cells from 3D culture. |
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| Keywords: | Recombinant human erythropoietin MCF-7 cell 2-dimensional MCF-7 cell culture 3-dimensional MCF-7 cell culture rHuEPO" },{" #name" :" keyword" ," $" :{" id" :" k0030" }," $$" :[{" #name" :" text" ," _" :" recombinant human erythropoietin MTT" },{" #name" :" keyword" ," $" :{" id" :" k0040" }," $$" :[{" #name" :" text" ," _" :" (4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay NRR" },{" #name" :" keyword" ," $" :{" id" :" k0050" }," $$" :[{" #name" :" text" ," _" :" neutral red retention TBE" },{" #name" :" keyword" ," $" :{" id" :" k0060" }," $$" :[{" #name" :" text" ," _" :" trypan blue exclusion AO/PI" },{" #name" :" keyword" ," $" :{" id" :" k0070" }," $$" :[{" #name" :" text" ," _" :" acridine orange/propidium iodide staining ULAT" },{" #name" :" keyword" ," $" :{" id" :" k0080" }," $$" :[{" #name" :" text" ," _" :" ultra-low adhesion technique FBS" },{" #name" :" keyword" ," $" :{" id" :" k0090" }," $$" :[{" #name" :" text" ," _" :" fetal bovine serum |
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