Characterization of Brucella abortus S19 as a challenge strain for use in a mouse model of brucellosis |
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| Authors: | Jessica M. Jacob Roy Curtiss |
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| Affiliation: | 1. College of Medicine and Dentistry, James Cook University, Queensland, 4811, Australia;2. College of Public Health, Medical and Veterinary Sciences, James Cook University, Queensland, 4811, Australia;3. Science & Technology, University of New England, New South Wales, 2351, Australia;1. Instituto de Patobiología, CICVyA-INTA (Instituto Nacional de Tecnología Agropecuaria), Nicolas Repetto y de Los Reseros s/n (B1686), Hurlingham, Bs. As., Argentina;2. Department of Veterinary Pathobiology, College of Veterinary Medicine & Biomedical Sciences, Texas A&M University, 4467 TAMU, College Station, TX 77843, USA;3. CONICET. Godoy Cruz 2290 (C1425) CABA, Argentina;4. Department of Molecular and Cellular Medicine, College of Medicine, Texas A&M University, College Station, TX 77843, USA;1. Department of Laboratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan;2. Department of Laboratory Medicine, Nagasaki University Hospital, 1-7-1 Sakamoto, Nagasaki, Japan;3. Department of Infectious Diseases, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan;4. Department of Respiratory Medicine, Nagasaki University Graduate School of Biomedical Sciences, 1-7-1 Sakamoto, Nagasaki, Japan;1. Department of Experimental Medicine, University of Rome “La Sapienza”, 00161, Roma, Lazio, Italy;2. Department of Emergency Medicine, University of Rome “La Sapienza”, 00161, Roma, Lazio, Italy;3. Department of Molecular Medicine, University of Rome “La Sapienza”, 00161, Roma, Lazio, Italy;1. Institute of Animal Medicine, College of Veterinary Medicine, Gyeongsang National University, Jinju 52828, Republic of Korea;2. Institute of Applied Sciences, Ho Chi Minh City University of Technology – HUTECH, 475A Dien Bien Phu St., Ward 25, Binh Thanh District, Ho Chi Minh City, Viet Nam;3. College of Veterinary Medicine, Chonbuk National University, Iksan 54596, Republic of Korea |
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| Abstract: | The objective of this project was to conduct a feasibility study to determine whether the Brucella abortus S19 vaccine infects and persists in mice and determine whether S19 can be used as a challenge strain for vaccine trial studies. Groups of BALB/c mice were inoculated (intraperitoneally, subcutaneously, intranasally) and euthanized to determine colonization titers in the spleens and lungs. This study showed that S19 does infect and persist in the tissues of mice for 8 weeks and demonstrates that S19 can be used, safely and economically under BSL2 containment, as the challenge strain for future trials to evaluate vaccine efficacy. |
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| Keywords: | Mice Vaccine Biosafety Bacterial loads Spleen |
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