In-vitro free radical scavenging effect and cytotoxic analysis of Black Cummins and Honey formulation |
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Authors: | Bharathi Rathi Sandhanasamy Devanesan Mohamad S. AlSalhi Amirtham J. Ranjith Singh |
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Affiliation: | 1. Department of Biochemistry, Shrimati Indira Gandhi College, Trichy, Tamil Nadu, India;2. Department of Physics and Astronomy, College of Science, King Saud University, P.O. Box, 2455, Riyadh 11451, Saudi Arabia;3. Department of Biotechnology, Prathyusha Engineering College, Chennai 600056, India |
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Abstract: | BackgroundThe antioxidant potential and antiproliferative activity of the extracts of Nigella sativa seeds (Black Cummins) and honey formulations are to be explored.MethodThe gas chromatography-mass spectrum (GC–MS) and Thin Layer Chromatography (TLC) fingerprint of Black Cummins and Honey formulation revealed alkaloid, saponin, volatile oil, flavonoid, glycosides, sugar, and phenolic compound in the extract. GC–MS profiling of the cold extract of Nigella sativa seeds and honey formulation shows peaks for eleven fractions of compounds. Using TLC, the phenolic compounds of Nigella sativa seeds and honey formulations were separated.ResultsThe current study discovers the cytotoxic effect of black Cummins seeds and honey formulation on human ovarian cancer (PA-1) cell line as assessed by MTT assay. PA-1 cells were inhibited with the increasing concentration of Nigella sativa seeds extract and honey formulation.ConclusionThe study validates the importance of the tested extracts in the treatment of cancer. |
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Keywords: | GC–MS Honey formulation Black cumin Anti-tumor Antioxidant |
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