Effects of soaking seeds in exogenous vitamins on active oxygen metabolism and seedling growth under low-temperature stress

This study investigated the influence of the exogenous application of vitamin B2 (V_B2), B12 (V_B12), biotin (V_H), and nicotinic acid (V_PP) on oxygen production in maize (Zea mays L.) seedlings at 5 °C for day 1, 3, 5 and 7. The seeds were soaked in V_B2, V_B12, V_H, and V_PP solutions for 24 h at the concentration of 100 mg/L, and control was soaked in distilled water. A total of 50 seeds were used for each treatment in germination boxes was repeated three times. The germination box was placed in a hypothermic incubator for 1, 3, 5, and 7 days in the dark at 5 °C, then moved to a plant growth room and kept for seven days. Compared with the V_H and V_PP treatments, the V_B2 and V_B12 treatments had higher thiobarbituric acid reactive substances, proline, and soluble sugars. The V_B2 and V_B12 treatments also increased the activities of superoxide dismutase (SOD), peroxidase (POD), catalase (CAT), and ascorbate peroxidase (APX) than other treatments. The V_B2 and V_B12 treatments reduced the contents of hydrogen peroxide (H₂O₂^–), superoxide anion (O₂^–), and the damage of reactive oxygen species (ROS) to cells, increased the stability of the cell membrane and the content of cell osmoregulation substances. Moreover, V_B2 and V_B12 had higher seedling growth, germination rate, and index. Treatments V_B2 and V_B12 could promote maize seed germination and growth under low-temperature stress. Exogenous vitamins in crop production can be a valuable tool for protecting plants against low-temperature stress.