Phytochemical evaluation and anticancer activity of rambutan (Nephelium lappaceum) fruit endocarp extracts against human hepatocellular carcinoma (HepG-2) cells |
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Authors: | Angalammal Perumal Mohamad S AlSalhi Sivakumari Kanakarajan Sandhanasamy Devanesan Rajesh Selvaraj Vairakannu Tamizhazhagan |
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Institution: | 1. Department of Zoology, Presidency College, Chennai 600005, India;2. Department of Physics and Astronomy, College of Science, King Saud University, P. O. Box 2455, Riyadh 11451, Saudi Arabia;3. Department of Zoology, Syed Ammal Arts and Science College, Ramanathapuram 623513, India |
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Abstract: | The current investigation was taken to screen the phytoconstituents present in fruit endocarp various extracts of Nephelium lappaceum commonly called as Rambutan fruit and its anticancer property against human hepatocellular carcinoma (HepG-2) cells. Different analytical techniques including qualitative phytochemical analysis, cell viability assay (MTT), apoptotic nuclear staining (DAPI), DNA fragmentation assay, Attenuated total reflection (ATR) and Gas chromatography–mass spectrometry (GC–MS) spectral analysis were carried out. ATR and GC–MS study revealed the presence of functional groups and 9 compounds, respectively in methanol endocarp extract. The results obtained depicts that methanol endocarp extract profoundly controlled cell proliferation and caused shrinkage of HepG-2 cells from polygonal to spherical shape. DAPI staining revealed that methanol endocarp extract caused increased fragmentation of nucleus and DNA fragmentation, which can be taken as a sign of apoptosis. The anticancer potential of methanol fruit endocarp extract of Nephelium lappaceum than other extracts and could be used successfully in future drug delivery systems and other biomedical concerns. |
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Keywords: | Phytochemicals Anticancer Apoptotic study ATR and GC–MS |
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