In vitro examination of anti-parasitic,anti-Alzheimer,insecticidal and cytotoxic potential of Ajuga bracteosa Wallich leaves extracts |
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Authors: | Muhammad Imran Hasnain Jan Shah Faisal Sajjad Ali Shah Sumaira Shah Muhammad Naeem Khan Muhammad Taj Akbar Muhammad Rizwan Faheem Jan Suliman Syed |
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Institution: | 1. Department of Botany, Government Post Graduate College Charsadda, KPK, Pakistan;2. Department of Biotechnology, Quaid-i-Azam University 45320, Islamabad, Pakistan;3. Department of Biotechnology, Bacha Khan University, Charsadda, KPK, Pakistan;4. Department of Botany, Bacha Khan University Charsadda, KPK, Pakistan;5. Department of Botany, Islamia College Peshawar, KPK, Pakistan;6. Department of Biotechnology and Genetic Engineering, Agriculture University KPK, Pakistan;7. Department of Microbiology, Abdul Wali Khan University, Mardan, KPK, Pakistan;8. Center for Biotechnology and Microbiology University of Swat, KPK, Pakistan;9. Programmatic Management of Drug Resistant T.B Unit Ayub Teaching Hospital, Abbotabad, Pakistan |
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Abstract: | This research study is mainly focused to evaluate the anti-parasitic, insecticidal, cytotoxic and anti-alzheimer potential of various leaf extracts of Ajuga bracteosa Wallich ex Bentham. 04 different extracts were prepared using solvent of different polarity to determine the best candidate for potent bioactivity i.e. n-hexane (NH), Ethyl acetate (EA), Ethanol (EL) and Chloroform (CH). Concentrations of each extracts were made specified for all activities. All extracts were exploited for broad range of biomedical applications including leishmaniasis, in vitro anti-Alzheimer, insecticidal and cytotoxic studies. Our results showed that A. bracteosa n-hexane extract was highly active against Leishmania Tropica with significant inhibition of 58 ± 1.61 for promastigote and 63 ± 2.29 for amastigote at 1000 μg/mL. Furthermore, promising anti-alzheimer activity acetylcholinesterase (AChE) 46 ± 0.83 and butrylcholineterase (BChE) 49 ± 1.17 was noted for n-hexane. The insecticidal potential of these extracts were test against five different insects (Rhyzopertha dominica, Trogoderma granarium, Tribolium castaneum, Sitophilus oryze, and Callosobruchus analis). The higest mortality rate of insecticidal activity was recorded by n-hexane followed by Ethyl acetate whereas ethanol extract was found to be less effective against all the test species. Significant cytotoxic potential of each plant sample against Artemia salina thus aware us for further detailed research to find out novel drugs. Based on our results we believe that Ajuga bracteosa could be used to develop as a potential botanical insecticide against different insect and pests, such as aphids as well as an excellent source for the compound isolation as anti-tumor agent. |
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Keywords: | Insecticidal Cytotoxic potential Leishmaniasis Phytochemicals Extracts |
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