MiRNAs regulate iron homeostasis in Paracoccidioides brasiliensis |
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| Authors: | Juliana S. de Curcio Lucas Nojosa Oliveira Mariana P. Batista Evandro Novaes Célia Maria de Almeida Soares |
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| Affiliation: | 1. Laboratório de Biologia Molecular, Instituto de Ciências Biológicas, Universidade Federal de Goiás, Campus II Samambaia, CEP: 74690-900, Goiânia, Goiás, Brazil;2. Departamento de Biologia, Universidade Federal de Lavras, Minas Gerais, CEP: 37200-000, Brazil;1. National Jewish Health, Denver, CO, USA;2. ?Iolani School, Honolulu, Hawai?i, USA;3. University of Colorado Anschutz Medical Campus, Aurora, CO, USA;4. Rocky Mountain Regional Veterans Affairs Medical Center, Aurora, CO, USA;1. Department of Immunology, National Institute of Infectious Diseases, 1-23-1 Toyama, Shinjuku-ku, Tokyo, 162-8640, Japan;2. Department of Life Science and Medical Bioscience, Waseda University, 2-2 Wakamatsucho, Shinjuku-ku, Tokyo, 162-8480, Japan;3. Department of Medical Technology, School of Human Sciences, Tokyo University of Technology, 5-23-22 Nishikamata, Ota-ku, Tokyo, 144-8535, Japan;1. State Key Laboratory of Infectious Disease Prevention and Control, National Center for AIDS/STD Control and Prevention, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Chinese Center for Disease Control and Prevention, Beijing 102206, China;2. Beijing Engineering Research Center of Protein and Antibody, Sinocelltech Ltd., Beijing 100176, China;1. Virology Unit, Viral Hepatitis Laboratory, Institut Pasteur du Maroc, Casablanca, Morocco;2. Faculté de Médecine de Casablanca, CHU Ibn Rochd, Casablanca, Morocco;3. Unité “Organisation Nucléaire et Oncogenèse”, INSERM U993, Institut Pasteur, Paris, France;1. Department of Medical Sciences, Section of Clinical Microbiology, Uppsala University, SE-751 85 Uppsala, Sweden;2. Department of Medical Sciences, Section of Infectious Diseases, Uppsala University, SE-751 85 Uppsala, Sweden;3. Department of Material Science and Engineering, Applied Materials Science, Uppsala University, SE-752 37 Uppsala, Sweden |
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| Abstract: | During pathogen interaction with the host, several mechanisms are used to favor or inhibit the infectious process; one is called nutritional immunity, characterized by restriction of micronutrients to pathogens. Several studies on fungi of the Paracoccidioides complex, have demonstrated that these pathogens remodel their metabolic pathways to overcome the hostile condition imposed by the host. However, molecular mechanisms that control the regulation of those metabolic changes are not fully understood. Therefore, this work characterizes the expression profile of miRNAs during iron deprivation and describes metabolic pathways putatively regulated by those molecules. Through analysis of RNAseq, 45 miRNAs were identified and eight presented alterations in the expression profile during iron deprivation. Among the differentially regulated miRNAs, five were more abundant in yeast cells during iron deprivation and interestingly, the analyses of genes potentially regulated by those five miRNAs, pointed to metabolic pathways as oxidative phosphorylation, altered in response to iron deprivation. In addition, miRNAs with more abundance in iron presence, have as target genes encoding transcriptional factors related to iron homeostasis and uptake. Therefore, we suggest that miRNAs produced by Paracoccidioides brasiliensis may contribute to the adaptive responses of this fungus in iron starvation environment. |
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| Keywords: | Paracoccidioidomycosis RNASEQ MICRORNA IRON |
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