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Biomedical and therapeutic potential of exopolysaccharides by Lactobacillus paracasei isolated from sauerkraut: Screening and characterization
Authors:Thiruvengadam Shankar  Selvam Palpperumal  Durairaj Kathiresan  Subbiah Sankaralingam  Chellapandian Balachandran  Kathirvelu Baskar  Abeer Hashem  Abdulaziz A. Alqarawi  Elsayed Fathi Abd_Allah
Affiliation:1. Vivekanandha College of Arts and Sciences for Women, Elayampalayam, Tamil Nadu, India;2. Saraswathi Narayanan College, Madurai Kamaraj University, Tamil Nadu, India;3. Department of Biotechnology, Thiagarajar College, Madurai-625 009, Tamil Nadu, India;4. National Centre of Excellence (MHRD), Thiagarajar College, Madurai-625 009, Tamil Nadu, India;5. Department of Ecotoxicology Ross Lifescience, Pvt., Ltd, Bhosari, Pune, India;6. Botany and Microbiology Department, College of Science, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia;7. Mycology and Plant Disease Survey Department, Plant Pathology Research Institute, ARC, Giza 12511, Egypt;8. Plant Production Department, College of Food and Agricultural Sciences, King Saud University, P.O. Box. 2460, Riyadh 11451, Saudi Arabia
Abstract:The intention of the study was evaluated for purification and characterization of exopolysaccharides from Lactobacillus paracasei; was isolated from homemade Sauerkraut sample collected from Sivakasi, Tamil Nadu, India, confirmed by biochemical and gene sequencing (16S rRNA). The purification and characterization of exopolysaccharides from candidate bacterium were studied on appearance, solubility of the EPS, carbohydrate estimation, emulsifying activity, sulphate, protein, uronic acid content, FTIR, HPLC and GC-MS analysis. The percentage of elemental carbon, (54.36%) hydrogen (21.74%), nitrogen (9.63%) and sulphur content (18.03%) were recorded in exopolysaccharides. The emulsification index (E24) of EPS was higher in toluene (79.20) and benzene (78.867) supplemented medium. FTIR spectrum of the candidate bacterial EPS confirmed presence of sulphate compounds, carboxyl group, and hydrogen bonded compounds etc. EPS exhibited 76.34% of Total Antioxidant Capacity (TAC), 71.15% of reducing power, 68.65% of Hydrogen Peroxide scavenging activity and also 60.31% DPPH radical scavenging activity. The potential antioxidant properties observed in exopolysaccharides from Lactobacillus paracasei is considered as valuable drugs.
Keywords:Exopolysaccharides (EPS)  FTIR  HPLC  Total antioxidant capacity (TAC)
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