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Circadian oscillations of RPCH gene expression in the eyestalk of the crayfish Cherax quadricarinatus |
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| Authors: | Martínez-Pérez Francisco Zinker Samuel Aguilar Guadalupe Valdés Jesús Aréchiga Hugo |
| Affiliation: | aPosgraduate School of Biological Sciences, Facultad de Ciencias, Universidad Nacional Autónoma de México, D.F., 04510 México, Mexico bDepartamento de Genética y Biología Molecular, CINVESTAV-IPN, Apartado Postal 14-740, D.F., 07000 México, Mexico cDepartment of Biochemistry, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Apartado Postal 14-740, D.F., 07000 México, Mexico dDivision of Research and Postgraduate Studies, Facultad de Medicina, Universidad Nacional Autónoma de México, D.F., 04510 México, Mexico |
| Abstract: | The RPCH and β-actin cDNAs from the crayfish Cherax quadricarinatus were amplified, cloned and sequenced. The primary structure sequences of these cDNAs were compared to other members of the AKH/RPCH family. Fluctuations in the amount of the C. quadricarinatus RPCH and β-actin mRNAs, as cDNAs, were quantified every 3 h by RT-PCR. Single cosinor analysis supports the notion of β-actin and RPCH mRNA circadian behavior in animals subjected to 12 h:12 h light/dark regimes. In constant darkness RPCH mRNA concentration changes to ultradian cycles. |
| Keywords: | Crayfish Crustacean Cherax quadricarinatus RPCH gene β-Actin gene Neurohormone Neuropeptide Circadian rhythm Ultradian rhythm |
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