Digestion of Collagen and Reticulin in Paraffin Sections by Collagenase |
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Authors: | James A. Green |
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Affiliation: | a Department of Anatomy, Indiana University School of Medicine, Indianapolis 7, Indiana |
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Abstract: | Tissues are fixed in ethanol or in Carnoy's 6:3:1 mixture and embedded in paraffin after routine ethanol dehydration. Sections are taken to water and then covered with 0.2 ml of a 0.9% NaCl solution containing 1 mg/ml of collagenase, and incubated at 50° C for 45 min. After this, they were washed and then stained by the usual methods for connective tissue fibers. Control sections were made by substituting plain 0.9% NaCl solution for the collagenase solution. The collagenase used was from bacteria and obtained from Nutritional Biochemicals Corporation, Cleveland 28, Ohio. |
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