Influence of accumulated phosphate on culture growth and formation of cinnamoyl putrescines in medium-induced cell suspension cultures of Nicotiana tabacum |
| |
Authors: | K-H Knobloch G Beutnagel J Berlin |
| |
Institution: | (1) Gesellschaft für Biotechnologische Forschung m.b.H., Mascheroder Weg 1, D-3300 Braunschweig, Federal Republic of Germany |
| |
Abstract: | The inorganic phosphate of the liquid nutrient medium was completely taken up by freshly inoculated cells of Nicotiana tabacum L. within the first 2 d of culture. Thus intracellular ortho-phosphate concentrations of approx. 0.06 M were accumulated, which upon growth of the cultures were diluted by cell division and subsequent cell growth. Cells from different stages of the growth cycle containing progressively decreasing levels of phosphate were transferred to a phosphate-free medium which normally stimulates the formation of cinnamoyl putrescines. The resulting accumulation of these compounds was inversely correlated with the intracellular phosphate level, whereas a direct linear relationship in the phosphate concentration was found with further growth in the phosphate-free medium.Abbreviations 2,4D
2,4-dichlorophenoxyacetic acid
- FW
fresh weight
- DW
dry weight
- MS-medium
Murashige-Skoog-medium (Murashige and Skoog 1962) |
| |
Keywords: | Cell culture Cinnamoyl putrescines Nicottana Phenolics Phosphate |
本文献已被 SpringerLink 等数据库收录! |