Regulation of phospholipase A(2) activity in cockroach (Periplaneta americana) fat body by hypertrehalosemic hormone: evidence for the participation of protein kinase C |
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Authors: | Sun D Steele J E. |
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Affiliation: | Department of Zoology, The University of Western Ontario, London, ON, N6A 5B7, Canada |
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Abstract: | Phospholipase A2 (PLA2) associated with the membrane fraction of trophocytes from Periplaneta americana fat body increases by as much as 100% when the cells are incubated with hypertrehalosemic hormone (HTH-II). Activation with HTH-II is approximately halved by inclusion of the PKC inhibitor sphingosine in the incubation medium. Because activation of PLA2 by HTH-II is blocked by the GDP analogue GDP-β-S, and the unactivated enzyme is activated by the GTP analogue GTP-γ-S it is likely that a G protein is involved in activation of the enzyme. Activation of PLA2 was also achieved by treating the trophocytes with the synthetic diacylglycerol 1-oleoyl-2-acetylglycerol in the presence of thapsigargin. This supports the view that protein kinase C is also involved in the activation process. |
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Keywords: | Calmodulin Cockroach Fat body G protein Hypertrehalosemic hormone Periplaneta americana Phospholipase A2 Protein kinase C |
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