Apolipophorin-III affects the activity of the haemocytes of Galleria mellonella larvae |
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Authors: | Zakarian Robert J. Dunphy Gary B. Albert Paul J. Rau Manfred E. |
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Affiliation: | a Department of Natural Resource Sciences, McGill University, 21, 111 Lakeshore Road, Ste. Anne de Bellevue, Québec, Canada H9X 3V9 b Biology Department, Concordia University, Montreal, Québec, Canada H3G 1M8 |
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Abstract: | Apolipophorin-III (apoLp-III) impaired the adhesion of plasmatocytes and a granular cell-subpopulation of larval Galleria mellonella to glass slides. The protein bound to haemocytes, limited the responses of the plasmatocytes to Bacillus subtilis and increased the percentage of a subgroup of granular cells with adhering bacteria. The total number of bacteria adhering to all the haemocytes on the slides declined. Injections of apoLp-III slowed bacterial removal from the haemolymph without affecting total haemocyte counts and impaired haemocyte attachment to glass slides. Purified apoLp-III bound to B. subtilis. ApoLp-III in serum bound to bacteria within 5 min, peaked at 15 min and was either shed or dissociated by 60 min. ApoLp-III bound to B. subtilis lowered the adhesion of the bacteria to the haemocytes and slowed the removal of the bacteria from the haemolymph. |
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Keywords: | Galleria Bacillus Haemocytes Adhesion Apolipophoria-III |
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