Comparative inhibition of nuclear RNA synthesis in cultured mouse leukemia L1210 cells by adriamycin and 4'-epi-adriamycin |
| |
Authors: | R G Wilson V Kalonaros M King R Lockwood M McNeill |
| |
Affiliation: | Department of Biochemistry, College of Medicine and Dentistry of New Jersey, New Jersey Medical School Newark, NJ 07103 U.S.A. |
| |
Abstract: | Adriamycin and 4'-epi-adriamycin were compared as to their effect on nRNA synthesis. 4'-Epi-adriamycin was a more effective inhibitor than the parent compound of RNA synthesis as measured by incorporation of [3H]-uridine. Adriamycin inhibited all three species of nRNA (ribosomal, non-poly(A)hnRNA, poly(A)hnRNA) to approximately the same extent. 4'-Epi-adriamycin on the other hand inhibited the nRNA species in the following order: non-poly(A)hnRNA greater than ribosomal RNA greater than poly(A)hnRNA. The inhibitory effects of both drugs on incorporation of uridine into RNA were reversible at low concentrations (5 microgram/ml). |
| |
Keywords: | PBS phosphate buffered saline hnRNA heterogeneous nuclear RNA |
本文献已被 ScienceDirect 等数据库收录! |