| Identification of up—regulated genes in human uterine leiomyoma by suppression subtractive hybridization |
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| 引用本文： | BINLI YONGLIANZHANG 等.Identification of up—regulated genes in human uterine leiomyoma by suppression subtractive hybridization[J].细胞研究,2002,12(3):215-221. |
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| 作者姓名： | BINLI YONGLIANZHANG |
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| 作者单位： | [1]StateKeyLaboratoryofMolecularBiology,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,320YueyangRoad,Shanghai200031,China [2]StateKeyLaboratoryofMole,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologicalSciences,ChineseAcademyofSciences,320YueyangRoad,Shanghai200031,China |
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| 摘 要： | In searching for differentially expressed genes in human uterine leiomyomas(Uls),suppression sub-tractive hybridization was used to construct an UL up-regulated library,which turned out to represent 88 genes.After two rounds of screening by reverse Northern analysis,twenty genes were proved to be up-regulated,including seventeen known genes and three genes with unknown function.All these genes were firstly associated with UL.Three genes with notable difference were selected for Northern confirmation.Our results proved the authenticity of the twenty genes.One gene named Phospholipase A2(PLA2) showed up-regulation in 4/6 of the patients and investigation of tissue distribution indicated that it had obvious expression in prostate,testis,liver,heart and skeletal muscle.
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| 关 键 词： | 子宫平滑肌瘤 增量调节基因 鉴定 抑制消减杂交 筛选文库 |
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