Medium and genotype factors influencing shoot regeneration from cotyledonary explants of Chinese cabbage (Brassica campestris L. ssp. pekinensis) |
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Authors: | F-L Zhang Y Takahata J-B Xu |
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Institution: | (1) Faculty of Agriculture, Iwate University, Morioka 020-8550, Japan Fax: 81-19-621-6177 e-mail: ytakahata@iwate-u.ac.jp, JP;(2) Beijing Vegetable Research Center, Beijing 100081, China, CN |
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Abstract: | Medium conditions for reliable shoot regeneration from cotyledonary explants of Chinese cabbage were examined. Maximum shoot
regeneration was obtained in the presence of 5 mg/l BA and 0.5 mg/l NAA. Shoot induction was further improved by the addition
of AgNO3 as well as higher concentrations (1.2–1.6%) of agar in the regeneration medium. When 123 genotypes were tested, a large variation
in regeneration frequency was observed, ranging from 95% to 0%. Shoot regeneration frequency was not related to origin and
days to maturity of the genotypes. Ethylene production from cultured explants seemed to play an important role in shoot regeneration.
Explants of highly responsive genotypes or if cultured on the medium solidified with a higher concentration of agar generally
showed low levels of ethylene production. However, AgNO3, which also enhanced shoot induction, resulted in an increase in ethylene production. The possible interaction between ethylene
and shoot regeneration is discussed.
Received: 26 September 1997 / Revision received: 6 March 1998 / Accepted: 20 March 1998 |
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Keywords: | Chinese cabbage (Brassica campestris L ssp pekinensis) Genotype Cotyledon culture AgNO3 Ethylene |
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