Alkaline extractability of pectic arabinan and galactan and their mobility in sugar beet and potato cell walls |
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Authors: | Agata Zykwinska, Corinne Rondeau-Mouro, Catherine Garnier, Jean-Fran ois Thibault,Marie-Christine Ralet |
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Affiliation: | aINRA, Unité de Recherche Biopolymères, Interactions, Assemblages BP 71627, 44316 Nantes Cedex 03, France |
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Abstract: | The organisation of sugar beet and potato cell walls was studied using alkaline extractions following a response surface methodology, simultaneously with solid-state 13C NMR spectroscopy. The influence of two extraction parameters: NaOH concentration (0.05, 0.275, 0.5 M) and temperature (40, 65, 90 °C) on the composition (neutral and acidic sugars) of the residues recovered was established. Treatments of increasing harshness progressively washed off non-cellulosic polysaccharides from the cell walls. Alkaline treatments applied to sugar beet cell wall material (SB-CWM) revealed the presence of diverse pectin populations. The existence of distinct pectin populations in potato cell wall material (P-CWM) was less outstanding. Solid-state 13C NMR applied to SB-CWM and P-CWM and residues after treatment by 0.275 M NaOH at 65 °C revealed two fractions of pectic arabinan and galactan side chains. One fraction was highly mobile, whereas the other one displayed restricted mobility. |
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Keywords: | Pectin Arabinan Galactan Xyloglucan Experimental design Solid-state 13C NMR spectroscopy |
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