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Excision of the 40kb segment of the TOL plasmid from Pseudomonas putida mt-2 involves direct repeats |
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| Authors: | Pierre Meulien Robert G Downing and Paul Broda |
| Institution: | (1) Department of Molecular Biology, University of Edinburgh, King's Buildings, Mayfield Road, EH9 3JR Edinburgh, UK;(2) Public Health Laboratory Service, Centre for Applied Microbiology and Research, Porton Down, SP4 OJG Salisbury, Wiltshire, UK;(3) Department of Biochemistry, UMIST, M60 1QD Manchester, UK |
| Abstract: | Summary The TOL plasmid pWWO gives rise to the Tol– plasmid pWWO-8 due to a specific excision event involving 40 kb of TOL DNA. This event occurs by means of reciprocal recombination involving a pair of directly repeated 1.4 kb sequences. This 40 kb segment is not involved as such in the formation in vivo of hybrid plasmids between TOL and resistance plasmids, notably RP4. |
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